Our Team

Member of :
Dr. Pramod Poudel
Head of the Department
9843810230
Pramod.poudel@nist.edu.np
Educational background
PhD in Biotechnology, Japan
M.Sc. in Biotechnology, Japan
M.Sc. Food Microbiology, National College, Affiliated to TU

Field(s) of specialization:

Basic and Applied microbial Biotechnology

·       Environmental microbiology

·       Microbial taxonomy

·       Fermentation technology

·       Bioprocess designing

·       Microbial community structure analysis of mixed culture system

·       Bioscience and biotechnology

Research interests:

·       Waste to wealth: Microbial transformation of human waste to liquid fertilizer

·       Studies on biotransformation of available waste biomasses to fine chemicals, fuels.

·       Studies on novel high temperature l-lactic acid fermentation.

·       Thermophilic microbes for enzyme production.

·       Isolation of not yet cultivated microbes from environment to understand the biogeochemical functions.

·       Environmental metagenomics.

Professional association:

1)     Life-member of Nepalese Society for Microbiology (NESOM), Nepal

2)     Life-member of Nepal biotechnology society, Nepal

3)     Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).

Skills in hand:

·       Matrix Assisted Laser Desorption/Ionization time of flight mass spectrometry (MALDI-TOF MS); High-performance liquid chromatography (HPLC); Gas Chromatography

·       Real-time PCR, 16S rRNA gene sequence; Denaturing gradient gel electrophoresis (DGGE)

·       Thin layer chromatography (TLC)

·       Scanning electron microscopy (SEM)

·       UV-VIS spectrophotometers

·       Fluorescence in-situ hybridisation (FISH)

·       CN corder, Freeze dryer

·       Atomic absorption spectroscopy (AAS)

·       Statistical tools (eg. XL-STAT), bioinformatics

Language skill

English, Nepali, Japanese, Hindi

Patent:

1.   “Kenji Sakai, Yukihiro Tashiro, Pramod Poudel?Hirokuni Miyamoto?Hisashi Miyamoto. L-lactic acid production directly from the starch using thermotolerant Bacillus sp. MC-07” , Japanese application patent No. 2014-194405, September, 2014

Scientific publications

1.   Pramod Poudel, Yukihiro Tashiro, Hirokuni Miyamoto, Hisashi Miyamoto, Yuki Okugawa, Sakai K. Development of a systematic feedback isolation approach for targeted strains from mixed culture systems. Journal of Bioscience and Bioengineering, Vol 123, 63–70, (2017).

2.   Yukihiro Tashiro, Inokuchi Shota, Pramod Poudel, Okugawa, Yuki Miyamoto, Hirokuni Miayamoto, Hisashi Miyamoto, Kenji Sakai. Novel pH control strategy for efficient production of optically active l-lactic acid from kitchen refuse using a mixed culture system, Bioresource Technology  vol. 216, 52– 59, (2016).

3.   Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. New application of Bacillus strains for optically pure l-lactic acid production: General overview and future prospects. Bioscience, Biotechnology, Biochemistry (BBB) Vol 80 (4), pp.642–654, (2016).

4.   Pramod Poudel, Yukihiro Tashiro, Hirokuni Miyamoto, Hisashi Miyamoto, Yuki Okugawa, Kenji Sakai. Direct starch fermentation to l-lactic acid by a newly isolated thermophilic strain, Bacillus sp. MC-07, Journal of Industrial Microbiology and Biotechnology, vol. 42 (1), pp. 143–149, (2015).

5.   Pramod Poudel, Hirokuni Miyamoto, Hisashi Miyamoto, Yuki Okugawa, Yukihiro Tashiro, Kenji Sakai. Thermotolerant Bacillus kokeshiiformis sp. nov. Isolated from marine animal resources compost. International Journal of Systematic and Evolutionary Microbiology, vol. 64 (8), pp. 2668–2674, (2014).

6.   Yukihiro Tashiro, Hiroko Matsumoto, Hirokuni Miyamoto, Yuki Okugawa, Pramod Poudel, Hisashi, Miyamoto, Kenji Sakai. A novel production process for optically pure l-lactic acid from kitchen refuse using a bacterial consortium at high temperatures.  Bioresource Technology, vol. 146, pp. 672–681, 2013.

7.   Saowanit Tongpim, Ratchanu Meidong, Pramod Poudel, Satoshi Yoshino,Yuki Okugawa, Yukihiro Tashiro, Masayuki Taniguchi, Kenji Sakai. Isolation of thermophilic l-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition. Journal of Bioscience and Bioengineering, vol.117, pp. 318–324, 2014.

8.   Pramod Poudel, Tika Bahadur Karki. Production of Probiotic Soy-Yoghurt by Inoculating Lactic Acid Bacteria isolated from Yoghurt Samples from Kathmandu Valley. Journal of Food Science and Technology Nepal, vol. 4, pp. 72–73, 2008.

9.   Vijay Singh Chhetri, Indra Bahadur Pachhai, Pramod Poudel, Huma Bokkhim, Yug Nath Dabadi. Antibiotic Resistant Escherichia coli in Meat Samples from Kathmandu Valley. Journal of Food Science and Technology Nepal, vol. 4, pp. 52–53, 2008.

10.Indra Bahadur Pachhai, Om Prakash Panta, Vijay Singh Chhetri and Pramod Poudel. Bacteriological analysis of drinking water of Kathmandu metropolitan city. Journal of Food Science and Technology Nepal, vol. 5, pp. 62–64, 2009.

 Book chapter:

1.   Kenji Sakai, Pramod Poudel & Yoshihito Shirai. Total Recycle System of Food Waste for Poly-L-lactic acid Output. In Advances in Applied Biotechnology. pp. 23-40, In-Tech d.o.o Open Access Publisher, www.intechopen.com; Croatia, ISBN 979-953-307-013-0, 2012.

  International conferences and Proceedings

1.   ?Pramod Poudel, Hiroko Matsumoto, Yuki Okugawa, Kenji Sakai. XIII International Congress of Bacteriology and Applied Microbiology, organized by International Union of Microbiological Societies (IUMS), Thermophilic Mixed Culture Fermentation of Optically Pure L-lactic Acid, pp. 191. Sapporo, Japan, September, 2011. (Poster, with peer-review)

2.   ?Pramod Poudel, Hiroko Matsumoto, Yuki Okugawa, Kenji Sakai. Proceeding on 5th International Symposium on the East Asian Environmental Problems (EAEP), Identification of thermo-tolerant Bacillus species from mixed culture system useful for the production of optically pure l-lactic acid, Fukuoka, Japan, pp. 271–275, November, 2011. (Oral, with peer-review)

3.   ?Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. Proceeding on 7th International Symposium on the East Asian Environmental Problems (EAEP), Feed-back isolation and identification of novel thermo-tolerant bacterial community structure within mixed-culture system, pp176–180, Fukuoka, Japan, November, 2013. (Oral, with peer-reviewed)

4.   ?Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. Mixed culture system for sustainable utilization of food waste biomass to produce value added products under thermophilic conditions. International Scientific Conference, Environmental Research, Issues, Challenges and Sustainable Development and Livelihood Security, pp. 32–34, Hotel Kasai, Lumbini, Nepal, November, 2014. (Oral, with peer-reviewed)

5.   ?N. Ishida, Y. Unoki-Kato, Pramod Poudel, Y. Tashiro, K. Sakai. Rapid microbial cell count in water sample using Flow cytometry. The 2nd Universiti Putra Malaysia–Kyushu Institute of Technology International Symposium on Applied Engineering and Sciences (SAES 2014), Tobata Campus, Kyushu Institute of Technology, Japan, 2014 (Oral, with peer-reviewed)

6.   ?F. S. Clament Chin, C. S. Vairappan, Pramod Poudel, Y. Tashiro, K. Sakai. Integrated utilization of wastes from palm oil industry by converting with functional microorganisms. The 2nd Universiti Putra Malaysia–Kyushu Institute of Technology International Symposium on Applied Engineering and Sciences (SAES 2014), Tobata Campus, Kyushu Institute of Technology, Japan, 2014. (Oral, with peer-reviewed)

7.   ?Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. Proceeding on 8th International Symposium on the East Asian Environmental Problems (EAEP), Mixed culture system for sustainable utilization of food waste biomass to produce value added products under high temperature, pp.170–174, Fukuoka, Japan, December, 2014. (Oral, with peer-reviewed)

8.   ? Pramod Poudel, Yukihiro Tashiro, Yuki Okugawa, Kenji Sakai. Feedback isolation and identification of not yet isolated thermophilic bacteria. International Joint Seminar on Microbial Study Useful for Establishing Sustainable Production System between Japan and Thailand, Kyushu University, Fukuoka, Japan, March 18, 2015.(Oral, with peer-reviewed)

9.   ?Yukihiro Tashiro, Pramod Poudel, Kenji Sakai. AFOB Regional Symposium 2014, High lactic acid production from renewable resources using thermophilic bacteria, Kuala Lumpur, Malaysia, pp. 47, February, 2014. (Invited oral, with peer-reviewed)

10.?Natsumi Ishida, Yoko Unoki-Kato, Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. Asian Congress on Biotechnology 2015, Development of rapid cell counting method by Flow cytometry. November 15-19, 2015, pp. 74 (EB18). Istana Hotel, Kuala Lumpur, Malasia. (poster, with peer-reviewed)

Domestic meetings and symposiums

1.   ?Pramod Poudel, Yuki Okugawa, Kenji Sakai & Hirokuni Miyamoto. Diversity of Thermotolerant Bacillus Species Useful For Producing l-Lactic Acid.., 48th Kagaku Kanren Shibu Godo Kyushu Taikai; 2011 July 9, pp. 192, Kitakyushu, Japan.(Poster)

2.   ?Pramod Poudel, Hiroko Matsumoto, Yuki Okugawa, Kenji Sakai. Optically pure l-lactic Acid Production via Thermophilic Fermentation Process. The proceedings on the 5th Young Scientist Seminar; Capacity Building and Development of Microbial potential and Fermentation Technology towards New era, 2011 November 22-23, pp. 87. Seminar Park, Yamaguchi, Japan. (Oral, with peer-reviewed)

3.   ?Pramod Poudel, Hiroko Matsumoto, Yuki Okugawa, Hirokuni Miyamoto, Kenji Sakai Annual Meeting of Japan Society for Bioscience, Biotechnology and Agrochemistry, Meta-fermentation: novel method for pure chemical production using of open mixed-culture system, Kyoto, March, 2012. (Oral, with peer-reviewed)

4.   ?Pramod Poudel, Yukihiro Tashiro, Maiko Yadomi, Hirokuni Miyamoto, Kenji Sakai. Annual Meeting of Japan Society for Bioscience, Biotechnology and Agrochemistry, Feed-back isolation and identification of thermotolerant bacteria within mixed culture system, Tokyo, March, 2014. (Oral, with peer-reviewed)

5.   ?Natsumi Ishida, Yoko Unoki-Kato, Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. Annual meeting, the society for Biotechnology, Japan, 2015. Development of rapid cell counting method by Flow cytometry (FCM). October 26-28, pp. 215 (2P-161). Kagoshima, Japan. (poster, with peer-reviewed)

6.   ?Yuko Ishibashi, Pramod Poudel, Hiroko Matsumoto, Yuki Okugawa, Yukihiro Tashiro, Hirokuni Miyamoto, Kenji Sakai. Annual meeting, the society for Biotechnology, Japan, 2015. Studies on direct high temperature lactic acid fermentation from non-gelatinized starch.  October 26-28, pp. 138 (1P-197). Kagoshima, Japan. (poster, with peer-reviewed).

7.   ?Clament Chin Fui Seung, Pramod Poudel, Hirokuni Miyamoto Yukihiro Tashiro, Kenji Sakai. 30th Annual Meeting of Japanese Society of Microbial Ecology (JSME2015, Oct 17-20). A novel bacterial species of yet unidentified genus isolated from thermophilic compost prepared from marine animal resources (MAR) materials. Poster number P-20; PG-091, Tsuchiura City Kijo Plaza, Tokyo, Japan. (poster, with peer-reviewed)

8.   ?Clament Chin Fui Seung, Pramod Poudel, Hirokuni Miyamoto Yukihiro Tashiro, Kenji Sakai. Annual Conference of Japan Society for Bioscience, Biotechnology and Agrochemistry, Characterization of a novel bacterium species isolated from thermophilic compost prepared from marine animal resources compost. March 27-31, pp. 54, No. 3F004, Saporro, Japan, 2016. (Oral, with peer-reviewed).

9.   Pramod Poudel, Yukihiro Tashiro, Kenji Sakai. One day symposium on Microbiology and One health concept in Nepal, 2016. Mixed culture fermentation system for total utilization of food waste biomass. Organised by Nepalese society for Microbiology (NESOM) and American Society for Microbiology (ASM), 3rd September, 2016, Hotel Orchid, Tripureswor, Kathmandu, Nepal. (Oral, without peer-reviewed)


Dr. Madhav Pd. Baral
principal
null
null@null
Mr. Om Prakash Panta
Lecturer (Coordinator M.Sc. Food Microbiology)
4360367
pantaop@gmail.com
Mr. Santosh Khanal
Lecturer (Coordinator B.Sc. Microbiology)
4360367
santoshkhanal007@gmail.com
Ms. Reena Pal
Lecturer
4360367
reenapal072@gmail.com
Ms. Asmita Kumal
Lab- Incharge
4360367
nist.asmi2017@gmail.com